How to get to know this grant
My research experiences made me very interested about finding a new opportunity related not only to the RBCs, but also to cellular physiology, specifically in the field of membranous channels. Discovering the description of this PhD program at the CNRS employment site, that I was following on Twitter, was for me like dreams coming true: the subject aims to determine the activity of cation channels in Red Blood Cells (RBCs) using techniques that was already practicing in my previous works like immunocytochemistry, Western Blot, cellular imaging, and flow cytometry.
Then came in the description the fact that this opportunity is part of ITN EVIDENCE, an EU program where there will be a collaboration between 15 ESRs in research laboratories and institutes originating from different European countries. And one of the things that attracted me the most to this program was that I will be receiving a continuous education comprising trainings and secondments in other institutes where I can acquire not only a multidisciplinary knowledge in the field that I will be working on in the next three years of my life but also where I can be widening my culture and intellectual abilities.
This program was therefore like a flow of blessings, so I applied immediately: I sent my application file to the ITN EVIDENCE site, to CNRS, and to my PhD director, Mr. Stéphane EGEE, just to make sure that my candidature is going to be seen. And for my good luck, I was interviewed by Dr. EGEE, and after that, the selection took place and I was the lucky chosen candidate for this position. Our laboratory is the UMR8227 at the "Station Biologique" in ROSCOFF-Brittany, France.
Reason for joining
I was particularly interested in the Red Blood Cells (RBCs) field due to a past experience I had at the REVIVA medical center in Lebanon in 2019 where I did an internship concerning the storage of the hematopoietic stem cells and the bone marrow transplantation. On the other hand, I have previously worked on the activity of calcium channels inside Glioblastoma cancer stem cells that are seeded on 3D scaffolds differing in their rigidities, at STIM laboratory in Poitiers-France where I did my Master2 internship (2020).
Now, I am an ESR6 PhD candidate who will be working on the hydration states of a RBC by determining the repertoire of channels involved in the life-ending in normal and pathological conditions. We will try to diagnose the risks imposed by cations permeability in stored, senescent, and ex-vivo generated erythrocytes. I will be performing electrophysiological techniques, notably the patch-clamp, in order to accumulate enough data concerning the behaviors of diverse ionic channels present on the membranes of these cells. Hence, during this PhD, I will grasp a deep knowledge about the physiology of a RBC, and the contribution of ionic channels to the awaited homeostasis.